cbse syllabus for class 12 biotechnology 2015

CLASS XII (THEORY)

One paper                                             Time: 3Hours            Total Marks : 70 (180 Periods) Unit V:  Protein and Gene Manipulation                                       Marks 40 (100 Periods) Chapter I: Recombinant DNA Technology                                                 15 Marks (40 Periods)

Introduction

Tool of rDNA Technology

Making rDNA

Introduction of Recombinant DNA into Host Cells

Identification of Recombinants Polymerase Chain Reaction (PCR) Hybridization Techniques

DNA Library

DNA Sequencing

Site-directed Mutagenesis

Chapter II: Protein Structure and Engineering                                 15 Marks (35 Periods)

Introduction to the World of Proteins

3-D Shape of Proteins

Structure-Function Relationship in Proteins

Purification of Proteins Characterization of Proteins Protein Based Products

Designing Proteins(Protein Engineering)

Chapter III: Genomics and Bioinformatics                                       10 Marks (25 Periods)

Introduction

Genome Sequencing Projects

Gene Prediction and Counting

Genome Similarity, SNPs and Comparative Genomics

Functional Genomics

Proteomics

History of Bioinformatics Sequences and Nomenclature Information Sources

Analysis using Bioinformatics Tools

Unit VI : Cell Culture and Genetic Manipulation  30 Marks (80 Periods)

Chapter I: Microbial Culture and Applications                                 10 Marks (26 Periods)

Introduction

Microbial Culture Techniques

Measurement and Kinetics of Microbial Growth

Scale up of Microbial Process Isolation of Microbial Products Strain isolation and Improvement

Applications of Microbial Culture Technology

Biosafety Issues in Microbial Technology

Chapter II: Plant Cell Culture and Applications                               10 Marks (27 Periods)

Introduction

Cell and Tissue Culture Techniques Applications of Cell and Tissue Culture Gene Transfer Methods in Plants Transgenic Plants with Beneficial Traits Biosafety in Plant Genetic Engineering

Chapter III: Animal Cell Culture and Applications                           10 Marks (27 Periods)

Introduction

Animal Cell Culture Techniques Characterisation of Cell Lines Methods of Gene Delivery into Cells Scale-up of Animal Culture Process Applications of Animal Cell Culture Stem Cell Technology

Tissue Engineering

Practicals

(60 Periods)

Note:  Every student will be required to do the following experiments during the academic session

List of Experiments

1.      Isolation of bacterial plasmid DNA and its detection by gel electrophoresis

2.      Restriction digestion of plasmid DNA and its analysis by gel electrophoresis

3.      Bacterial transformation using any plasmid

4.      Data retreival and data base search using internet site NCBI

5.      Download a DNA and protein sequence from internet, analyse it and comment on it.

6.      Cell viability assay

7.      Determination of blood groups

8.      Estimation of DNA

9.      Ion-exchange chromatography for proteins.

10.    Reading of DNA sequencing gel to arrive at the sequence

11.    Estimation of blood glucose by enzymatic method (GOD/POD)

12.    Project work

Scheme of Evaluation:

Time: 3 Hours                                                                                        Max. Marks 30

The scheme of evaluation at the end of the session will be as under:

A.     Two experiments                :                6+6 (only one computer based practical) Practical record                                          :                04

Viva on Practicals               :                04

B.      Project work                      :

Write up                             :                05

Viva on project                   :                05

Total 30

Recommended Books :

1.      A Text Book of Biotechnology - Class XI : Published by CBSE, New Delhi

2.      A Laboratory Manual of Biotechnology - Class XI : Published by CBSE, New Delhi

3.      A Text Book of Biotechnology - Class XII : Published by CBSE, New Delhi

4.      A Laboratory Manual of Biotechnology - Class XII : Published by CBSE, New Delhi